Natural Product and Metabolomics

Natural Product and Metabolomics Mass Spectrometry is often become very useful comes into the final confirmation stage of a chemical structure. This is because by noting the fragmentation pattern and the total molecular mass of compound, researchers will be able to confirm the compound’s chemical formula, followed by confirming the chemical structure proposed based on previous instrumental analyses. Nowadays, Gas Chromatography and Liquid Chromatography are coupled with mass spectrometry in order for researchers to obtain the molecular mass right after the compounds are separated chromatographically. However, GC-MS are more often being used to study natural product compared to LC-MS because LC-MS doesn’t have its database constructed like GC-MS due to the extensive range of secondary metabolites and it is hardly reproducible. (Lee Yoon et al., 2013) Researchers can confirm the compound by matching the molecular mass obtained from GC-MS with the established database, while LC-MS users will have to refe r to the data provided by previous researchers on that particular field of study. There are a lot of journals regarding the studies of primary and secondary metabolites being published and a lot of researches will insert their sample for GC-MS experiment and then compared their compound’s fragmentation pattern and also molecular weight to the National Institute of Standards and Technology libraries, which will tells researchers the structure of their compounds, even if there are no exact matching, researches will at least have a rough idea on their structure. In the early 1990s, Solid-phase microextraction (SPME) was introduced to extract compounds from samples without the use of solvent; this method is later applied on samples before undergoing GC-MS process. This technique enables researchers to extract compounds from sample in a much simpler and cheaper way since it doesn’t include the use of solvent. SPME works by adsorbing compounds into its fibre coated absorbing phase from the sample, and then inserted into GC for desorption of compound into it and then separated. (Dagostino Sanz et al., 2014) This technique is so convenient that researchers can even carry it along when going into a jungle to adsorb compounds from plant of interest and bringing it back to the lab to undergo GC-MS experiment, much more convenient than plucking a big bunch of plants sample to the lab for solvent soaking. Samples can be injected into GC-MS through either split of splitless injection; split injection system will direct most of the samples out to the atmosphere and only allow approximately 1% of it to enter the column while splitless injection system allows most of the samples to enter the column. Split/splitless system are designed to give reproducible chromatographic retention times and also to keep the chromatographic requirements of column in place, to use split of splitless, it is depending on the sensitivity required. Though splitless system allows more sample to be injected in however, metabolomics researches are better be carried out with split system because metabolites are present in wide range concentrations and it allows researchers to analyze volatile compounds eluting near the solvent peak. (Cyril Jousse, 2009) Besides plant’s metabolites, GC-MS is also very useful for bio-metabolics, some of the bio-metabolics researches are for example Chrysoula Christou et al. did a research on analysis of organic acids in human urine in clinical settings in order to develop a method for organic acid profiling in human urine, this can be useful to detect metabolics or other health disorders. Their sample was being separated by gas column and undergo quadrupole mass spectrometer in the electron ionization (EI) mode and as a result chromatogram and mass spectra shown 11 organic acids separated and identified by conducting similarity search on NIST library (3). A research was carried out by Ioannis Papoutsis and the team of research. where GC-MS method was used to determine amisulpride in blood. Amisulpride is an antipsychotic drugs which is prescribed to psychiatric patients, overdosing can cause arrhythmia, bradycardia and hypotension. (4) SPME was first used by them to extract compounds from the b lood samples and then separated by using gas chromatography and finally detected by mass spectrometer, results show that GC-MS can be used to monitor amisulpride in the blood and this can be used in pharmacokinetic studies and also forensic investigation. Archeology GC-MS is also used in assisting archaeology studies; it is especially useful when comes to determining the chemical constituents of the sample. In one of the recent archaeology research, M. Mà ©nager, C. Azà ©mard and C. Vieillescazes studied the mummification balms from the mummy skulls with FTIR and GC-MS. Twelve mummified heads were collected, the balms on the heads were extracted with organic solvent and water before introduced to GC-MS. Molecular components were eluted by using helium, and injected with split system. Samples were eluted by using helium as carrier gas with the flow of 1mL.min-1 and oven temperature was set to: 50  °C for 2 min, 50–250  °C at 8  °C ·min−1, 250–350  °C at 3  °C ·min−1, and 350  °C during 20 min. 1 ÃŽ ¼L of each sample was injected with a splitting system and the injection temperature was set at 250  °C. Mass spectra were generated by electron ionization mode with electron ionization energy of 70 eV, an ionization time of 25,000 ÃŽ ¼s and a mass range of 40–650 m/z. (5) As a result, those mummification balms were found to contain mixtures such as oils, fats, beeswax, resins, proteins, polysaccharides and minerals, these compounds are confirmed again by matching mass spectra with NIST library. With the use of only FTIR without confirmation by GC-MS, researchers will only be able to know the functional group in it without confirming the compounds. Cosmetic In cosmetic industries, chemical compounds in every cosmetic must be carefully studied as it’s applied onto human’s skin and body, therefore any mistake made will cause allergy or irritation to end users. Synthetic husk has been widely applied in cosmetic industry due ti its long-lasting odor, however, it was found that two groups of synthetic musk can cause many health disorders. Many countries have also set up regulations on the amount of synthetic musk in order to protect end users, this has become another parameter in quality control of cosmetic products. In Beijing, China, Haifeng Dong and his team of researchers conducted a research on 7 synthetic musk in cream by using SPME and then GC-MS, cream is chosen as it is the most common form of cosmetic product. (6) Cosmetic creams contains moisturizing, nutritional agent and also many lipophilic compounds. Synthethic musks have low polarity and it is also one of lipophilic compounds, which cause synthethic musks can be hardly be separated out from other liphophilic compounds. Cream samples must undergo extra cleanup steps such as by matrix solid phase dispersion method (MSPD) in order minimizes the matrix effect. Since water and isopropanol was used to separate synthetic musks from lipophilic compounds, supported liquid extraction will need to be used in order absorb water and remove interferences to give better purification. Tandem MS was used in this research in order to improve the selectivity and decrease the detection limit. This research which deals with dream shows slightly different in the preparation method compared to other GC-MS research, however, overall concept is still similar.

Telecommunication Industry Analysis

The domestic telecommunication industry offers a broad range of services through four primary product segments: radio, television, voice communications, and broadband services, the two largest being voice communications and broadband services (Industry). These services are offered to businesses and personal consumers, and are the industry’s primary user segments. Companies in the telecommunication industry are the primary means by which communication is provided, whether it is by telephone, Internet, or television. Using transmissions of signals over networks of radio towers, data is transferred to customers using devices, such as a phone (Telecommunications). Determining which segment to market to and who drives the market is integral for companies in the telecommunication industry to understand. User Segments The telecommunication industry markets to two segments: business and personal consumers. The business segment utilizes the services of the telecom industry for better communication, higher efficiency, and better distribution of data (Telecommunications and Business). As businesses require communication devices for email, phone, teleconferencing, and data transferring, companies are marketing to businesses offering the most advanced and efficient products. Businesses also allow for a larger budget for spending, and consequently telecommunication companies are refining their product to fit their needs on a larger scale. AT&T and Verizon Communications are the leading providers in the business segment, offering services such as videoconferencing, networking, Internet, and voice communications. Personal consumers are the other segment the telecommunication industry markets to. With over 290 million subscribers in the United States (U.S.), companies in the telecommunication industry are on a very competitive playing field. AT&T and Verizion, again the leading providers in the personal consumer segment, spend a large amount each year on advertising trying to reach their markets (The Account). Consumers want products with the most speed and reliability, which forces companies to update constantly. With new products and features coming out regularly, it is imperative these companies stay current with the latest technology. Product Segments The two product segments that define the telecommunication industry are voice communications and broadband services. Voice communications entail phone services such as phone calls and voice messaging. Businesses and personal users rely heavily on this service, as people are requiring reliable and efficient phone service in their everyday lives. Features play an integral role in defining the product, so companies must have feedback from consumers to know how to improve their product (Cell Phone). Broadband services offer Internet access to its users. This technology is improving constantly, becoming faster and more efficient. Integrating into phones, television, and DSL, broadband services are becoming increasingly important to having a reliable product and reaching customers (Industry). Over the past decade, Internet subscribers have doubled, reaching over 266 million customers (World), which shows the prevalence of broadband services in the United States. As many of the telecommunication companies have merged in recent years, they are able to offer a larger product as well as â€Å"bundled media packages†, which brings in new revenue sources and opportunities for growth (Industry). In the telecommunication industry, the consumer is driving the market. As technology is advancing and more features are becoming available, companies must customize their products to fit the consumers’ needs. Many consumers require different features and options, which require the companies to stay current with recent trends. Customer service has also played a large role in the satisfaction of customers, and the companies take a lot of feedback to improve their products (Industry). Trends in the Telecommunication Industry As the telecommunication industry evolves, so do the trends that drive it. Demographics have become less important, although age and occupation does play a major role (Market). Teens are becoming a target for companies, as well as business professionals (Teens). Consumer trends are driving adoption of phones in the market, as social networking, jobs, and personal use are becoming parts of everyday life (Consumer). Technological advancements are playing a major role in the development of the telecommunication industry. Products are becoming faster, cheaper, and of higher quality, as well as including features and applications for consumers so they can communicate and work more efficiently (Top). Competitors One of the top competitors in the telecommunications industry is AT&T. With over $124 billion in total revenue (T), AT&T is one of the largest providers of telecommunication services in the United States. Their main products are for businesses as well as the consumer, including wireless, broadband, phone, and data solution services. AT&T’s strategy involves working with its suppliers and customers to improve their efficiencies and form a positive, long-lasting relationship with them. They improve their company by becoming more sustainable, in hopes of promoting a positive impact on the communities it serves (AT&T Corporate). Verizon Communications is also a leading competitor in the telecommunications industry. With $106.5 billion in total revenue last year (VZ), Verizon is another leading provider of telecommunication services in the United States. Verizon offers products similar to AT&T, which enables them to compete closely with AT&T. Their basic strategy involves being proactive in improving their services to satisfy customers. Verizon continually adapts to new technology and brings innovation to all its products. Their goal is to improve the quality of life for its customers by offering innovative products (Verizon). Sprint Nextel Communications, totaling $32 billion in total revenue in 2010 (S), is a smaller competitor but is still among the top companies in the domestic telecommunication industry. Sprint offers phones for business and personal use, as well as business solutions to help the company operate its communications with more ease. Part of their strategy is to foster and develop relationships with organizations to form alliances and deliver integrated products and services. Sprint is also involved in becoming more responsible, in regards to its products, the environment, and its customers (About).

Stepper Motors and Servomotors

Stepper motors and Servomotors Motors are the special electrical devices that have been developed by the engineers for the conversion of energy from one form to another. Engineers have developed the science of motors to an extent wherein they can be used for numerous applications and in several technologies. We would discuss the characteristics of two of the most commonly used types of motors, i. e. the stepper motors and the servomotors. Both these motors serve different purposes and are used and employed for different reasons and different applications based on the need and the requirement.The stepper motors are those motors that work on the basis of the x/y axis coordinates and works on the basis of a special torch, which can help in pointing at all the directions and the synchronization observed between the two coordinates and axes of the motor makes it simple to work. The best characteristic of the stepper motor is that it offers high reliability and not only reliability but als o exhibits extremely high speed, thus making the operations much more efficient. Numerous technologies make use of the stepper motors and that is because they are also inexpensive.Since these motors make sure that they don’t miss the steps, they give and lead to extremely high efficiency and output. The costs and efforts required for the management and repair of these motors are also comparatively less. Servo motors are slightly different as compared to the stepper motors. They are actually the rotary actuators that help in gaining complete accuracy and precision for the purpose of controlling the angular position during the working of the motors. They are high performance motors and usually replace the stepper motors due to their high performances.One major advantage that the stepper motors have but servomotors don’t have is that the stepper motors are comparatively cheaper than the servomotors and hence tend to be more employed and used. Servo motors are also usually bigger and heavier than the stepper motors and are hence used mostly for heavy machinery and big equipments. One thing that amazes users is the intelligence factor that can be embedded for the servomotors. Servomotors can be made interesting and much more automated with certain developments and coding.They are commonly use for different applications including that of robotics and automated manufacturing. By comparing both these types of motors, we see that they both have their own characteristics and their own pros and cons. Though their working dynamics differ significantly, they still can be used at the same place. The final decision however lies on the requirement and the need for the efficiency, cost and size of the motor. Industries make use of both these motor types very frequently. Reference link: http://classof1. com/homework-help/engineering-homework-help

Essay on Treatment Of African Americans 1865-1895

During the span of thirty years from 1865 to 1895 blacks that lived within this time frame went through arguably the most profound series of events to occur in African American history. Southern blacks were faced with prejudice, bondage, slavery, and ultimately survival. Shortly after the thirteenth amendment was ratified, stating that: â€Å"Neither slavery nor involuntary servitude, except as a punishment for crime whereof the party shall have been duly convicted, shall exist within the United States, or any place subject to their jurisdiction.† The southern governments enacted a series of Black Codes that were purposefully meant to keep blacks â€Å"as near to a state of bondage as possible.† Blacks could not bear arms, be employed in†¦show more content†¦In other words, it put teeth in the Thirteenth Amendment.† By Congress passing the Civil Rights Act by a two-thirds majority over President Johnson’s veto, it was the first time in American history that legislation became law over the president. This was a victory politically and socially for the blacks in America. Even though whites in the north were sympathetic towards abolishing slavery, they still did not want blacks to take part in the northern schools or neighborhoods. Also the idea of blacks being able to vote was still far and few between. During the next year in June 1866 Congress submitted yet another amendment to the constitution. The Fourteenth Amendment granted citizenship to â€Å"All persons born or naturalized in the United States, and subject to the jurisdiction thereof, are citizens of the United States and of the State wherein they reside.† This solidified the black’s right to citizenship on a political scale, yet they still did not have the right to vote. The Amendment did not reduce segregation or stop most southern states from continuing to persecute the black population but it did give passageway for the Reconstruction Acts to take place. These acts forced the southern states to be split into military districts until they complied with Congress. 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